![]() ![]() With either form of sequencing, whole-exome sequencing or whole genome sequencing, some have argued that such practices should be done under the consideration of medical ethics. One must also keep in mind that non-coding regions can be involved in the regulation of the exons that make up the exome, and so whole-exome sequencing may not be complete in showing all the sequences at play in forming the exome. Especially for single nucleotide variants, whole genome sequencing is more powerful and more sensitive than whole-exome sequencing in detecting potentially disease-causing mutations within the exome. On the other hand, whole genome sequencing has been found to capture a more comprehensive view of variants in the DNA compared to whole-exome sequencing. In addition, since the exome only comprises 1.5% of the total genome, this process is more cost efficient and fast as it involves sequencing around 40 million bases rather than the 3 billion base pairs that make up the genome. This distinction is largely due to the fact that phenotypes of genetic disorders are a result of mutated exons. It has also been found to be more effective than other methods such as karyotyping and microarrays. Sequencing an individual's exome instead of their entire genome has been proposed to be a more cost-effective and efficient way to diagnose rare genetic disorders. Next-gen sequencing includes both whole-exome sequencing and whole-genome sequencing. This compares to former methods which may have taken months. ![]() This technology is starting to become more common in healthcare and research not only because it is a reliable method of determining genetic variations, but also because it is cost effective and allows researchers to sequence entire genomes in anywhere between days to weeks. ![]() Next-generation sequencing (next-gen sequencing) allows for the rapid sequencing of large amounts of DNA, significantly advancing the study of genetics, and replacing older methods such as Sanger sequencing. Different cell types only transcribe portions of the exome, and only the coding regions of the exons are eventually translated into proteins. As a result, the entirety of the exome is not translated into protein in every cell. While the exome is constant from cell-type to cell-type, the transcriptome changes based on the structure and function of the cells. It is important to note that the exome is distinct from the transcriptome, which is all of the transcribed RNA within a cell type. See also: List of human protein-coding genes 1, List of human protein-coding genes 2, List of human protein-coding genes 3, and List of human protein-coding genes 4 ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |